저출력 레이저조사와 염증성 자극물질이 치은섬유아세포의 유전자 발현에 미치는 영향에 관한 실험적연구
AN EXPERIMENTAL STUDY ON THE EFFECT OF LOW LEVEL LASER AND SOME CYTOKINES ON GENE EXPRESSION OF HUMAN GINGIVAL FIBROBLASTS
김정민, 신금백,
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김정민 ( ) - 서울대학교 치과대학 구강진단·구강내과학교실
신금백 ( ) - 전북대학교 치과대학 구강진단·구강내과학교실
KMID : 0355219940190020057
Abstract
Gingival fibroblasts were cultured and subjected to the test of Northern blot analysis for the demonstration of various mRNA expression in response to the low level laser treatment. For duplication of in vivo. wound healing process, fibroblasts were pretreated with proinflammatory cytokine interleukin-1l (IL-IQ) or mitogenic substance phorbol 12-myristate 13-acetate(PM A) prior to laser irradiation.
The results were as follows
1. By the laser irradiation, the gene.expression of collagen type I was markedly increased in gingival fibroblasts,especially in the ? caw of PMA pretreatment. The gene expression of collagen typ IV, however, was not only affected by laser irradiation but also by chemical cell stimulation.
2 Oncogene v-myc expression was affected by both Laser inadiation and IL-10 or PMA stimulation. But v-fos
gene expression was not detected in any case of this experimental system.
3 Heat shock gene(Hsp 70) was expressed constitutively, but slightly increased by laser irradiation.
4 mRNA of fibroblast growth factor(FGF) was induced by both laser irradiation and IL-1B or PMA treatment.
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